Antibacterial efficacy of endodontic irrigation solutions against Enterococcus faecalis

Methods Forty-one extracted single-rooted teeth were included in the present study. After content removal and autoclaving, they were divided into eight groups among which two were used as positive and negative controls. The remaining six groups were instrumented and irrigated with solutions containing 17% EDTA, sterile saline solution, and different concentrations of NaOCl. In addition, chlorhexidine 2% was also used in three groups. Microbiological evaluation was performed after 30 minutes, and 24 hours, respectively. E faecalis strain ATCC 29212 was used for culture tests. Statistical analysis was performed using non-parametric tests.